在“EditPlus.exe”或"EditPlus64.exe"所在的目录下找到"editplus_u.ini"文件（如果不存在就新建一个），修改这个文件即可更改EditPlus的主题。修改完之后，回到EditPlus软件，选择“工具”->“设置配置文件目录”，把第一个选项设置为"editplus_u.ini"所在的目录即可（如图所示）。
下面给出一种黑色主题的配色方案，可以直接复制替换掉原来"editplus_u.ini"的内容。（更多主题方案可以百度“editplus 主题”）
[Options] Placement=2C0000000000000001000000FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF70100002F010000E306000010040000FF Window List=00000000000000000000000000000000FF Marker List=00000000000000000000000000000000FF Function List=00000000000000000000000000000000FF Open Remote=5C020000550100006404000067030000FF Expand=00000000000000000000000000000000FF Project Pos=00000000000000000000000000000000FF Workspace Path=D:\Program Files (x86)\EditPlus Cliptext Window=224 Cliptext Window 2=489 Output=90 Output 2=170 Folding=1 FindX=586 FindY=429 Find=align Tab Index=1 Cliptext=2 Custom colors=6D6D7200FFFFFF00FFFFFF00FFFFFF00FFFFFF00FFFFFF00FFFFFF00FFFFFF0031282700FFFFFF00FFFFFF00FFFFFF00FFFFFF00FFFFFF00FFFFFF00FFFFFF00FF Matching Brace=1 Indent guide=1 Cliptext Edit=00000000000000000000000000000000FF [Files] Encoding=65001 Backup=0 Backup Remote=0 [Fonts] Edit Window=E3FFFFFF00000000000000000000000090010000000000010302013143006F007500720069006500720020004E0065007700000000000000000000000000000000000000000000000000000000000000000000000000000000000000FF Printer=F4FFFFFF00000000000000000000000090010000000000010000003143006F007500720069006500720020004E0065007700000000000000000000000000000000000000000000000000000000000000000000000000000000000000FF Output Window=F4FFFFFF00000000000000000000000090010000000000010000003143006F007500720069006500720020004E0065007700000000000000000000000000000000000000000000000000000000000000000000000000000000000000FF Hex Viewer=F4FFFFFF00000000000000000000000090010000000000010000003143006F007500720069006500720020004E0065007700000000000000000000000000000000000000000000000000000000000000000000000000000000000000FF Custom 1=F4FFFFFF00000000000000000000000090010000000000010000002241007200690061006C000000720020004E0065007700000000000000000000000000000000000000000000000000000000000000000000000000000000000000FF Custom 4=F5FFFFFF0000000000000000000000009001000000000001000000224D0053002000530061006E0073002000530065007200690066000000000000000000000000000000000000000000000000000000000000000000000000000000FF [Tool Option] Top Selector=1 [Colors\Text] Background=2238503 Default=0 Foreground=16777215 [Colors\Keyword 1] Foreground=8272368 Default=0 [Colors\Embedded script] Foreground=16777215 Default=0 [Colors\Keyword 3] Foreground=16777215 Default=0 [Colors\Keyword 6] Foreground=8716287 Default=0 [Colors\Keyword 7] Foreground=8716287 Default=0 [Colors\Keyword 8] Foreground=8716287 Default=0 [Colors\Keyword 9] Foreground=8454143 Default=0 [Colors\Keyword 10] Foreground=8716287 Default=0 [Colors\Quotation] Foreground=8454143 Default=0 [Colors\Quotation 2] Foreground=5107956 Default=0 [Colors\Line comment] Foreground=10789024 Default=0 [Colors\Line number] Foreground=12632256 Background=2238503 Default=0 [Colors\Folding mark -] Background=2238503 Default=0 [Colors\Matching words] Default=0 Background=718314 [Colors\Keyword 2] Foreground=16379142 Default=0 [Colors\Folding mark +] Background=2238503 Default=0 [Colors\Text selection] Background=7039851 Default=0 [Colors\Number] Foreground=16524240 Default=0 [Colors\Block comment] Foreground=8421504 Default=0 [Colors\Keyword 5] Foreground=15574913 Default=0 [Colors\Ruler] Foreground=12632256 Default=0 Background=2566187 [Colors\Cursor indicator] Background=16777215 Default=0 [Colors\Indent Guide] Foreground=7499117 Default=0