r - DESeqDataSetFromHTSeqCount 无法识别我的目录

Question

我目前正在尝试对从 HTSeq 收到的一个计数文件运行 DESeqDataSetFromHTSeqCount。出于某种原因，DESeqDataSetFromHTSeqCount 似乎找不到我的目录，我收到此错误：

Error in file(file, "rt") : cannot open the connection
In addition: Warning message:
In file(file, "rt") :
  cannot open file 'C:/Users/me/Documents/genomes//0': No such file or directory

当我运行 getwd() 时，文件位置是正确的。我不知道 /0 表示什么并且我没有想法。任何建议都会有所帮助！！

我的运行代码是：

directory <- "C:/Users/me/Documents/genomes/" 
setwd(directory) 

countData <- read.csv("HTSeqoutputgene.txt", header = TRUE, sep = "\t") 

outputPrefix <- "SRR11_DESeq2"

sampleFiles<- countData

condition <- "Treated"

sampleTable <- data.frame(sampleName = sampleFiles[1],
                          fileName = sampleFiles[2],
                          condition = condition)

dim(sampleTable) sampleTable$condition <- factor(sampleTable$condition)


ddsHTSeq <- DESeqDataSetFromHTSeqCount(sampleTable = sampleTable,
                                       directory = directory,
                                       design= ~ condition)

提前致谢

标签： rseqrna-seq